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Anti-PODXL Monoclonal Antibody-AF647

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Catalog No. TMAZ-0170A6
Alias Podocalyxin-like protein 1, Podocalyxin, Gp200, GCTM-2 antigen

Anti-PODXL Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting PODXL. Anti-PODXL Monoclonal Antibody-AF647 can be used in ELISA, FACS.

Anti-PODXL Monoclonal Antibody-AF647

Anti-PODXL Monoclonal Antibody-AF647

😃Good
Catalog No. TMAZ-0170A6Alias Podocalyxin-like protein 1, Podocalyxin, Gp200, GCTM-2 antigen
Anti-PODXL Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting PODXL. Anti-PODXL Monoclonal Antibody-AF647 can be used in ELISA, FACS.
Pack SizePriceAvailabilityQuantity
25 μg$25012 days
50 μg$41512 days
100 μg$62012 days
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Product Introduction

Bioactivity
Description
Anti-PODXL Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting PODXL. Anti-PODXL Monoclonal Antibody-AF647 can be used in ELISA, FACS.
AliasPodocalyxin-like protein 1, Podocalyxin, Gp200, GCTM-2 antigen
Ig Type
Rabbit monoclonal IgG
Reactivity
Human
Verified Activity
Flow cytometry analysis of PODXL overexpressed 377F cells with TMAZ-0170A6, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line).
Application
ELISA, FACS
Recommended Dose
0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted
ConstructionThis antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of PODXL protein.
PurificationProtein A Affinity Purified
AppearanceLiquid
Formulation0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin.
Purity> 95% as determined by SDS-PAGE.
Research BackgroundInvolved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells
Related Conjugates and Formulations
Conjucates
AF647
Others Formats
Biotin/APC/PE/AF488/AF555
Antibody Types Available
2
Antigen Details
Immunogen
PODXL
Antigen Species
Human
Gene ID
5420
Uniprot ID
Biology Area
Drug Target
Chemical Properties
Molecular Weight150 kDa
Stability & Storage
Stability & StorageFor unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze.
TransportFor unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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