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Anti-VCAM1 Monoclonal Antibody-AF555
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Catalog No. TMAZ-0012A5
Alias Vascular cell adhesion protein 1, INCAM-100
Anti-VCAM1 Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting VCAM1. Anti-VCAM1 Monoclonal Antibody-AF555 can be used in ELISA, FACS.
Anti-VCAM1 Monoclonal Antibody-AF555
Anti-VCAM1 Monoclonal Antibody-AF555
😃Good
Catalog No. TMAZ-0012A5Alias Vascular cell adhesion protein 1, INCAM-100
Anti-VCAM1 Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting VCAM1. Anti-VCAM1 Monoclonal Antibody-AF555 can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $320 | 12 days | |
| 50 μg | $535 | 12 days | |
| 100 μg | $800 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-VCAM1 Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting VCAM1. Anti-VCAM1 Monoclonal Antibody-AF555 can be used in ELISA, FACS. |
| Alias | Vascular cell adhesion protein 1, INCAM-100 |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of VCAM1 overexpressed 400F cells with TMAZ-0012A5, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Single-pass type I membrane |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of VCAM1 protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed: 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed: 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed: 22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed: 35210567) |
| Conjucates | AF555 |
| Others Formats | Biotin/APC/PE/AF488/AF647 |
| Antibody Types Available | 4 |
| Immunogen | VCAM1 |
| Antigen Species | Human |
| Gene ID | 7412 |
| Uniprot ID | |
| Biology Area | Immunology Research |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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