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Anti-CD33 Monoclonal Antibody-AF555
Anti-CD33 Monoclonal Antibody-AF555
Anti-CD33 Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting CD33. Anti-CD33 Monoclonal Antibody-AF555 can be used in ELISA, FACS, IF.
Catalog No. TMAZ-0119A5
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Anti-CD33 Monoclonal Antibody-AF555
Catalog No. TMAZ-0119A5Alias Sialic acid-binding Ig-like lectin 3, Myeloid cell surface antigen CD33, gp67
Anti-CD33 Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting CD33. Anti-CD33 Monoclonal Antibody-AF555 can be used in ELISA, FACS, IF.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose. | Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $285 | 12 days | |
| 50 μg | $475 | 12 days | |
| 100 μg | $715 | 12 days |
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CD33 Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting CD33. Anti-CD33 Monoclonal Antibody-AF555 can be used in ELISA, FACS, IF. |
| Alias | Sialic acid-binding Ig-like lectin 3, Myeloid cell surface antigen CD33, gp67 |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of CD33 overexpressed 340F cells with TMAZ-0119A5, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS, IF |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Single-pass type I membrane |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of CD33 protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed: 10611343, PubMed: 11320212, PubMed: 15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed: 7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed: 10887109, PubMed: 28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed: 10206955, PubMed: 10556798, PubMed: 10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed: 10206955, PubMed: 10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed: 15597323) |
| Conjucates | AF555 |
| Others Formats | Biotin/APC/PE/AF488/AF647 |
| Antibody Types Available | 3 |
| Immunogen | CD33 |
| Antigen Species | Human |
| Gene ID | 945 |
| Uniprot ID | |
| Biology Area | Cancer Research |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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