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Anti-DCD Monoclonal Antibody-AF647

Catalog No. TMAZ-0265A6
Alias Preproteolysin, Dermcidin

Anti-DCD Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting DCD. Anti-DCD Monoclonal Antibody-AF647 can be used in ELISA, FACS.

Anti-DCD Monoclonal Antibody-AF647

Anti-DCD Monoclonal Antibody-AF647

Catalog No. TMAZ-0265A6Alias Preproteolysin, Dermcidin
Anti-DCD Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting DCD. Anti-DCD Monoclonal Antibody-AF647 can be used in ELISA, FACS.
Pack SizePriceAvailabilityQuantity
25 μg$57012 days
50 μg$94512 days
100 μg$1,41512 days
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Product Introduction

Bioactivity
Description
Anti-DCD Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting DCD. Anti-DCD Monoclonal Antibody-AF647 can be used in ELISA, FACS.
AliasPreproteolysin, Dermcidin
Ig Type
Rabbit monoclonal IgG
Reactivity
Human
Verified Activity
Flow cytometry analysis of DCD overexpressed 472F cells with TMAZ-0265A6, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line).
Application
ELISA, FACS
Recommended Dose
0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationSingle-pass type I membrane
ConstructionThis antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of DCD protein.
PurificationProtein A Affinity Purified
AppearanceLiquid
Formulation0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin.
Purity> 95% as determined by SDS-PAGE.
Research BackgroundFound in sweat, has an antimicrobial activity during early bacterial colonization (PubMed: 11694882, PubMed: 23426625). The secreted peptide assembles into homohexameric complexes that can associate with and also insert into pathogen membranes (PubMed: 23426625). Once inserted in bacteria membranes forms anion channels probably altering the transmembrane potential essential for bacterial survival (PubMed: 23426625). Highly effective against E.coli, E.faecalis, S.aureus and C.albicans (PubMed: 11694882). Optimal pH and salt concentration resemble the conditions in sweat (PubMed: 11694882). Also exhibits proteolytic activity, cleaving on the C-terminal side of Arg and, to a lesser extent, Lys residues (PubMed: 17448443)|Promotes survival of neurons and displays phosphatase activity (PubMed: 9736629). It may bind IgG (PubMed: 9736629)
Related Conjugates and Formulations
Conjucates
AF647
Others Formats
Biotin/APC/PE/AF488/AF555
Antibody Types Available
1
Antigen Details
Immunogen
DCD
Antigen Species
Human
Gene ID
117159
Uniprot ID
Biology Area
Other Functions
Chemical Properties
Molecular Weight150 kDa
Stability & Storage
Stability & StorageFor unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze.
TransportFor unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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