Deacetylase Inhibitor Cocktail (100× in 70% DMSO)

• Deacetylase Inhibitor Cocktail-Instruction-Manual(2.48 MB)

1.Avoid multiple freeze/thaw cycles. It is recommended to aliquot and store the samples at -80℃ for future use.

2.Lysis buffer or culture medium containing the deacetylase inhibitor cocktail should be prepared and used immediately. They are not suitable for freezing and storage for later use.

3.Sodium Butyrate in the components has an odor that persists when contaminated. Centrifuge before opening to prevent residual liquid from contaminating the environment.

4.Different cell types have varying levels of endogenous enzymes. Please adjust the volume of the required cocktail accordingly.

5.This product is for R&D use only, not for diagnostic procedures, food, drug, household, or other uses.

6.Please wear a lab coat and disposable glove.

• Thaw the product at room temperature and centrifuge at low speed before opening the vial to ensure any liquid adhering to the tube walls is collected at the bottom. Add 10µL of deacetylase inhibitor cocktail per milliliter of sample directly to the culture medium, cell lysis buffer, or tissue extraction fluid. If adding to cell culture medium, incubation time of 16-48 hours is recommended before assaying.
• This product can be applied in Western Blotting (WB), ImmunoFluorescence (IF) and Flow Cytometry (FCM) and etc.

• Accurate Ingredients —— Accurate components and clear concentrations to avoid experimental uncertainty.
• Comprehensive Protection —— Efficiently inhibit various deacetylases and comprehensively protect acetylated proteins from degradation.
• Cost-Effective —— Lower price with higher quality.