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Deacetylase Inhibitor Cocktail (100× in 70% DMSO)
Catalog No. C0048
Protein acetylation is a common posttranslational modification that plays a crucial role in regulating biological processes such as gene expression, cell proliferation, and metabolic regulation. The acetylation level of proteins within cells is regulated by both acetyltransferases and deacetylases. Deacetylase inhibitors can effectively prevent the loss of acetylation modifications to increase the acetylation level.
TargetMol deacetylase inhibitor cocktail consists of four components that effectively inhibit the activity of various deacetylases and maintain protein acetylation status, including reversible inhibitors for Class I/II deacetylases such as Trichostatin A and Sodium Butyrate, a reversible inhibitor for Class III deacetylases (sirtuins, SIRTs) like Nicotinamide, and a selective reversible inhibitor for SIRT1, known as EX-527. To enhance the detection of protein acetylation level, deacetylase inhibitor cocktail can be added to cell culture medium for a certain incubation period before cell collection and protein extraction.
All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.TargetMol deacetylase inhibitor cocktail consists of four components that effectively inhibit the activity of various deacetylases and maintain protein acetylation status, including reversible inhibitors for Class I/II deacetylases such as Trichostatin A and Sodium Butyrate, a reversible inhibitor for Class III deacetylases (sirtuins, SIRTs) like Nicotinamide, and a selective reversible inhibitor for SIRT1, known as EX-527. To enhance the detection of protein acetylation level, deacetylase inhibitor cocktail can be added to cell culture medium for a certain incubation period before cell collection and protein extraction.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 1 mL | $36 | In Stock | |
| 10 mL | $172 | In Stock | |
| 50 mL | $675 | In Stock | |
| 100 mL | $1,088 | In Stock |
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Composition
| Ingredient | CAS | MW | Concentration (100x) | Target |
|---|---|---|---|---|
| Trichostatin A | 58880-19-6 | 302.374 | 40 μM | Class I/II HDAC |
| Sodium Butyrate | 156-54-7 | 110.088 | 200 mM | Class I/II HDAC |
| Nicotinamide | 98-92-0 | 122.125 | 400 mM | Class III HDAC |
| EX-527 | 49843-98-3 | 248.708 | 1 mM | SIRT |
Features
- Accurate Ingredients —— Accurate components and clear concentrations to avoid experimental uncertainty.
- Comprehensive Protection —— Efficiently inhibit various deacetylases and comprehensively protect acetylated proteins from degradation.
- Cost-Effective —— Lower price with higher quality.
Handling Instruction
- Thaw the product at room temperature and centrifuge at low speed before opening the vial to ensure any liquid adhering to the tube walls is collected at the bottom. Add 10µL of deacetylase inhibitor cocktail per milliliter of sample directly to the culture medium, cell lysis buffer, or tissue extraction fluid. If adding to cell culture medium, incubation time of 16-48 hours is recommended before assaying.
- This product can be applied in Western Blotting (WB), ImmunoFluorescence (IF) and Flow Cytometry (FCM) and etc.
Notice
1.Avoid multiple freeze/thaw cycles. It is recommended to aliquot and store the samples at -80℃ for future use.
2.Lysis buffer or culture medium containing the deacetylase inhibitor cocktail should be prepared and used immediately. They are not suitable for freezing and storage for later use.
3.Sodium Butyrate in the components has an odor that persists when contaminated. Centrifuge before opening to prevent residual liquid from contaminating the environment.
4.Different cell types have varying levels of endogenous enzymes. Please adjust the volume of the required cocktail accordingly.
5.This product is for R&D use only, not for diagnostic procedures, food, drug, household, or other uses.
6.Please wear a lab coat and disposable glove.
Instruction Manual
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