Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)

• Compatible with Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
• Thaw in Room Temperature. Please centrifuge it at low speed before opening the cover, so as to throw the liquid adhered to the wall of the tube to the bottom.
• Add concentrated cocktail at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
• Briefly vortex cocktails to help facilitate the dissolution.
• Extracts of up to 20 g wet weight.

• Accurate Ingredients —— Accurate components and clear concentrations to avoid experimental uncertainty
• Comprehensive Protection—— Effectively inhibit various proteases to protect proteins from being degraded
• Cost-Effective —— Lower price with higher quality

• Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
• Some DUB proteases (one example is ATAXIN-3) cannot be suppressed by traditional protease inhibitors, such as E-64, AEBSF, bestatin, leupeptin and Aprotinin.

(Reference: Neil D. Rawlings, Guy Salvesen et al. Handbook of Proteolytic Enzymes, Vol.1, 2012.)