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Protease Inhibitor Cocktail, MS-safe (10×)
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Protease Inhibitor Cocktail, MS-safe (10×)

Catalog No. C0055
The production and breakdown of intracellular proteins is in dynamic equilibrium under stable environmental conditions and when proteins are extracted from cells and tissues in vitro, crude cell extracts contain many endogenous enzymes that are capable of degrading the target proteins, e.g. phosphatases and proteases. These enzymes are released from the cells or activated, leading to the breakdown of the target protein and affecting the results of the analysis. To avoid this, one way to increase the yield of target proteins is to incorporate inhibitors of the relevant enzymes.
TargetMol Mass Spectrometry-Compatible Protease Inhibitor Cocktail has a solvent of ddH2O and consists of four components including Aprotinin, Bestatin, E-64 and Leupeptin, which have broad specificity for serine and cysteine proteases and aminopeptidases, and no EDTA. This product is free of AEBSF, which avoids the potential for peak drift in mass spectra. This product does not contain AEBSF, thus avoiding the possible drift of the peaks in the mass spectra. Therefore, protein samples extracted with this product can be used for Mass Spectrometry (MS) detection and analysis, including proteomics.
TargetMol Protease Inhibitor Cocktail protects your proteins conveniently and reliably by efficiently inhibiting a wide range of proteases from animal, plant, bacterial, yeast, and fungal samples. It is suitable for extracting proteins from a wide range of tissues and cells to obtain target proteins more efficiently.
All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Pack SizePriceAvailabilityQuantity
1 mL$26In Stock
1 mL * 10$188In Stock
1 mL * 50$758Backorder
1 mL * 100$1,088Backorder
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Handling Instruction | TargetMol Composition

Ingredient CAS MW Concentration (10x) Target Type
Aprotinin 9087-70-1 6511.44 0.08 mM serine proteases Reversible
Bestatin hydrochloride 65391-42-6 344.84 5 mM aminopeptidase B; LAP Reversible
E-64 66701-25-5 357.41 1.5 mM cysteine proteases Irreversible
Leupeptin Hemisulfate 103476-89-7 475.59 2 mM cysteine proteases; serine proteases Reversible

Handling Instruction | TargetMol Handling Instruction

  • Thaw in Room Temperature. Please centrifuge at low speed before opening the cover,so as to throw the liquid adhering to the tube wall to the bottom.
  • Dilute at a 1:10 (v/v) ratio into the solution sample (e.g., cell lysate or tissue extract) and briefly vortex to accelerate dissolution.
  • Applicable for: MS, WB, Co-IP, pull-down, IF, IHC, kinase assay, etc.

Handling Instruction | TargetMol Storage

  • Store at -20℃ for up to 1 year; store at 2-8℃ for up to 2 months.
  • Avoid repeated freeze-thaw cycles and aliquoting is recommended.

Handling Instruction | TargetMol Notice

1.This product does not contain EDTA. If needed, please prepare it separately. If activity of metalloproteinases in the extraction solution will be analyzed, adding EDTA is not recommended.

2.Lysis buffer containing Cocktail should be freshly prepared before use. It is not recommended to freeze and store for future use.

3.The endogenous enzyme levels vary among different cell types. Please adjust the volume of Cocktail accordingly.

4.The product is for R&D use only, not for diagnostic procedures, food, drug, household or other uses.

5.Please wear a lab coat and disposable gloves