Streptavidin Agarose 6FF

The Streptavidin-Biotin (SA-Biotin) system has a wide range of applications in the biological field due to its extremely high binding affinity (K = 10^-15). TargetMol's Streptavidin Agarose 6FF is an affinity chromatography medium in which Streptavidin is attached to agarose beads and can be used for the purification of biotin or biotinylated proteins, antibodies and more. Due to the extremely high affinity between streptavidin and biotin, denaturing conditions are usually required for elution. Streptavidin, on the other hand, has a weaker affinity for iminobiotin, binds in the pH 9.5-11.0 range, and elutes at pH 4.0 without the need for denaturants, thus better preserving the activity of the affinity coupler.

TargetMol's Streptavidin Agarose 6FF uses a highly cross-linked 6% agarose matrix that allows it to withstand high flow rates and has good chemical stability, making it ideal for large-scale purification.