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DKK1 Protein, Human, Recombinant (hFc)

Catalog No. TMPY-05479
DKK1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 52.5 kDa and the accession number is I1W660.
Pack SizePriceAvailabilityQuantity
100 μg$4627-10 days
1 mg$3,0007-10 days
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Product Information

Biological Activity
Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.01-0.07μg/mL in the presence of 10 ng/mL of mouse Wnt3a.
Description
DKK1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 52.5 kDa and the accession number is I1W660.
Species
Human
Expression System
HEK293 Cells
TagC-hFc
Accession NumberI1W660
Synonyms
SK,DKK-1,dickkopf WNT signaling pathway inhibitor 1
Construction
A DNA sequence encoding the human DKK1 (NP_036374.1) (Met2-His266) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Thr 32
Protein Purity
> 95 % as determined by SDS-PAGE.
Molecular Weight52.5 kDa (predicted)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

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