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ENO1 Protein, Human, Recombinant (His)

Catalog No. TMPY-02412

ENO1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 49.3 kDa and the accession number is P06733-1.

ENO1 Protein, Human, Recombinant (His)

ENO1 Protein, Human, Recombinant (His)

Catalog No. TMPY-02412
ENO1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 49.3 kDa and the accession number is P06733-1.
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100 μg$255In Stock
200 μg$4367-10 days
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Product Information

Biological Activity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
ENO1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 49.3 kDa and the accession number is P06733-1.
Species
Human
Expression System
E. coli
TagN-His
Accession NumberP06733-1
Synonyms
PPH,NNE,MPB1,HEL-S-17,enolase 1, (α),enolase 1, (alpha),ENO1L1
Construction
A DNA sequence encoding the human ENO1 (P06733-1) (Met 1-Lys 434) was expressed, with a polyhistide tag at the N-terminus. Predicted N terminal: Met
Protein Purity
> 90 % as determined by SDS-PAGE
ENO1 Protein, Human, Recombinant (His)
Molecular Weight49.3 kDa (predicted); 47 kDa (reducing conditions)
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 10% glycerol, pH 8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
The ENO1 gene encodes a multifunctional enzyme that has been identified as a key component of the glycolytic pathway. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. The results of the ENO1 expression profiling of ovarian follicles suggest that ENO1 may play an important dual role in the progress of follicular development, where ENO1 acts as a glycolytic enzyme and also mediates apoptosis. ENO1 overexpression could make the primary culture follicle granulosa cells in vitro improve progesterone secretion. The over-expression of ENO1 protein can enhance the abilities of proliferation and migration in gastric cancer cells of AGS, which indicates that ENO1 may be an important potential tumor-marker associated with the development of gastric cancer. ENO1 and GPI can be used as markers of human sperm freezability before starting the cryopreservation procedure. The inhibition of ENO1 expression may be a novel strategy for therapy for NHLs patients, and it may be a target for drug resistance.

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