FAP Protein, Mouse, Recombinant (His)

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FAP Protein, Mouse, Recombinant (His)

Catalog No. TMPK-00923

Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells. FAP Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 86.4 kDa and the accession number is P97321-1.

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Pack Size	Price	Availability
100 μg	$523	7-10 days
500 μg	$2,090	7-10 days
1 mg	$3,490	7-10 days

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Biological Description

Biological Information	Immobilized Mouse FAP, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).
Description	Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells. FAP Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 86.4 kDa and the accession number is P97321-1.
Species	Mouse
Expression System	HEK293 Cells
Tag	N-His
Accession Number	P97321-1
Synonyms	FAPα,FAPalpha,DPPIVA,APCE,SIMP,FAP,DPPIV,FAPA,Seprase
Construction	Leu26-Asp761
Protein Purity	> 95% as determined by Tris-Bis PAGE
Molecular Weight	86.4 kDa (predicted). Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.

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