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GPA34/VSIG1 Protein, Human, Recombinant (His)

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GPA34/VSIG1 Protein, Human, Recombinant (His)

Catalog No. TMPK-01076

VSIG1, a cell adhesion protein of the immunoglobulin superfamily, is preferentially expressed in stomach, testis, and certain gastric, esophageal and ovarian cancers. Here, we describe the expression patterns of three alternatively spliced isoforms of mouse Vsig1 during pre- and postnatal development of stomach and potential function of Vsig1 in differentiation of gastric epithelia. VSIG1 is required for the establishment of glandular versus squamous epithelia in the stomach. GPA34/VSIG1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 24 kDa and the accession number is Q86XK7-1.

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Pack Size	Price	Availability
100 μg	$418	7-10 days
500 μg	$1,670	7-10 days
1 mg	$2,800	7-10 days

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Biological Description

Description	VSIG1, a cell adhesion protein of the immunoglobulin superfamily, is preferentially expressed in stomach, testis, and certain gastric, esophageal and ovarian cancers. Here, we describe the expression patterns of three alternatively spliced isoforms of mouse Vsig1 during pre- and postnatal development of stomach and potential function of Vsig1 in differentiation of gastric epithelia. VSIG1 is required for the establishment of glandular versus squamous epithelia in the stomach. GPA34/VSIG1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 24 kDa and the accession number is Q86XK7-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q86XK7-1
Synonyms	MGC44287,1700062D20Rik,Glycoprotein A34,VSIG1,Gpa34,dJ889N15.1
Construction	Val22-Glu232
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	24 kDa (predicted). Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	VSIG1, a cell adhesion protein of the immunoglobulin superfamily, is preferentially expressed in stomach, testis, and certain gastric, esophageal and ovarian cancers. Here, we describe the expression patterns of three alternatively spliced isoforms of mouse Vsig1 during pre- and postnatal development of stomach and potential function of Vsig1 in differentiation of gastric epithelia. VSIG1 is required for the establishment of glandular versus squamous epithelia in the stomach.