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HSP70 Protein, Human, Recombinant (His)

Catalog No. TMPY-02443

HSP70 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 72.2 kDa and the accession number is A8K5I0.

HSP70 Protein, Human, Recombinant (His)

HSP70 Protein, Human, Recombinant (His)

Catalog No. TMPY-02443
HSP70 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 72.2 kDa and the accession number is A8K5I0.
Pack SizePriceAvailabilityQuantity
100 μg$386In Stock
200 μg$6607-10 days
500 μg$2,0807-10 days
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Product Information

Biological Activity
1. Measured by its ability to bind human PARP1 in a functional ELISA. 2. Measured by its ability to bind mouse PARP1 in a functional ELISA.
Description
HSP70 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 72.2 kDa and the accession number is A8K5I0.
Species
Human
Expression System
Baculovirus Insect Cells
TagN-His
Accession NumberP0DMV8
Synonyms
HSPA1,HSP72,HSP70I,HSP70-1A,HSP70-1,HEL-S-103,heat shock 70kDa protein 1A
Construction
A DNA sequence encoding the human HSPA1A (NP_005337.2) (Ala2-Asp641) was expressed, with a polyhistidine tag at the N-terminus. Predicted N terminal: His
Protein Purity
> 85 % as determined by SDS-PAGE
HSP70 Protein, Human, Recombinant (His)
Molecular Weight72.2 kDa (predicted)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
HSPA1A is a member of the Hsp70 protein family. The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. HSP are abundant and conserved proteins present in all cells. Upon temperature shock or other stress stimuli, HSP is synthesized intracellularly, which may protect cells from protein denaturation or death. Extracellularly, HSP can serve a cytokine function to initiate both innate and adaptive immunity through activation of APC. HSP serves also a chaperone function and facilitates the presentation of antigen peptide to T cells. Molecular chaperones of the Hsp70 family have diverse functions in cells. They assist the folding of newly synthesized and stress-denatured proteins, as well as the import of proteins into organelles, and the dissociation of aggregated proteins. The well-conserved Hsp70 chaperones are ATP dependent: binding and hydrolysis of ATP regulate their interactions with unfolded polypeptide substrates, and ATPase cycling is necessary for their function. All cellular functions of Hsp70 chaperones use the same mechanism of ATP-driven polypeptide binding and release.

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