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IgG2 Protein, Human, Recombinant (S257A) is expressed in HEK293 mammalian cells. The predicted molecular weight is 25 kDa and the accession number is P01859.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $256 | 7-10 days | |
1 mg | $1,180 | 7-10 days |
Biological Activity | Measured by its ability to bind human CD32a-His in a functional ELISA. |
Description | IgG2 Protein, Human, Recombinant (S257A) is expressed in HEK293 mammalian cells. The predicted molecular weight is 25 kDa and the accession number is P01859. |
Species | Human |
Expression System | HEK293 Cells |
Tag | Tag Free |
Accession Number | P01859 |
Synonyms | Immunoglobulin heavy constant γ 2,Immunoglobulin heavy constant gamma 2,IgG2 |
Construction | A DNA sequence encoding the human IgG2 Fc region (P01859) (Glu 99-Lys 327, 257Ser/Ala) was expressed and purified. Predicted N terminal: Glu 99 |
Protein Purity | > 90 % as determined by SDS-PAGE |
Molecular Weight | 25 kDa (predicted); 34 kDa (reducing condition, due to glycosylation) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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