LRIG1 Protein, Human, Recombinant (His)

Catalog No. TMPK-01143

LRIG1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 83 kDa and the accession number is Q96JA1-1.

LRIG1 Protein, Human, Recombinant (His)

Catalog No. TMPK-01143

LRIG1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 83 kDa and the accession number is Q96JA1-1.

Pack Size	Price	Availability
100 μg	$487	7-10 days
500 μg	$1,950	7-10 days
1 mg	$3,250	7-10 days

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User Guide of Recombinant Proteins

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Product Information

Biological Activity	Immobilized Human LRIG1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-LRIG1 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.
Description	LRIG1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 83 kDa and the accession number is Q96JA1-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q96JA1-1
Synonyms	LRIG1,LIG-1,LIG1,Img,D6Bwg0781e
Construction	Ala35-Ser779
Protein Purity	> 95% as determined by Tris-Bis PAGE
Molecular Weight	83 kDa (predicted). Due to glycosylation, the protein migrates to 85-105 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM MES, 150 mM NaCl, 1 mM EDTA (pH 5.0). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in 50mM MES, 150mM NaCl, 1mM EDTA (pH 5.0). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The leucine-rich repeats and immunoglobulin-like domains (LRIG)-1 is a tumor suppressor gene that belongs to the LRIG family. LRIG1 expression has prognostic significance in various human cancers. Somatic mutations, which are associated with a certain rate of response to targeted therapies, are ubiquitously found in human non-small cell lung cancer (NSCLC). LRIG1 was an independent prognostic factor for OS of NSCLC patients. LRIG1 in combination with other clinicopathological risk factors was a stronger prognostic model than clinicopathological risk factors alone.