1. Loaded LDLR Protein, Human, Recombinant (mFc) (Cat#TMPY-05323) on ProA Biosensor, can bind PCSK9 Protein, Human, Recombinant (His) (Cat#TMPY-00895) with an affinity constant of 16 nM as determined in BLI assay (Sartorius Octet RED384). 2. Immobilized Anti-PCSK9 (Amg)-IgG2 Antibody at 2 μg/mL (100 μL/well) can bind PCSK9 Protein, Human, Recombinant (His) (Cat#TMPY-00895), the EC50 is 14-52 ng/mL.

PCSK9 Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The predicted molecular weight is 72.5 kDa and the accession number is Q8NBP7.

Human

HEK293 Cells

Proprotein convertase subtilisin/kexin type 9,PCSK9,NARC1

A DNA sequence encoding the human PCSK9 (NP_777596.2) (Met1-Gln692) was expressed with a polyhistidine tag at the C-terminus.

≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC.

A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Human Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) is a secretory subtilase belonging to the proteinase K subfamily. PCSK9 is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the ER , the pro domain and mature chain secrete together through noncovalent interactions. PCSK9 binds with low-density lipoprotein receptor (LDLR) and plays a major regulatory role in cholesterol homeostasis. Inhibition of PCSK9 function by preventing PCSK9/LDLR interaction is currently being explored as a means of lowering cholesterol levels. PCSK9 also binds to apolipoprotein receptor 2 (ApoER2), and play a role in the neural development.