PEDF Protein, Mouse, Recombinant (His)

Catalog No. TMPY-01112

PEDF Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.8 kDa and the accession number is P97298.

PEDF Protein, Mouse, Recombinant (His)

Catalog No. TMPY-01112

PEDF Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.8 kDa and the accession number is P97298.

Pack Size	Price	Availability	Quantity
50 μg	$462	7-10 days

Bulk & Custom

Add to Cart

Questions

Resource Download

User Guide of Recombinant Proteins

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Information

Biological Activity	Measured by its binding ability in a functional ELISA. Immobilized mouse SERPINF1-His at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CSNK2A1 with a linear range of 0.31-2.5 μg/ml.
Description	PEDF Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.8 kDa and the accession number is P97298.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P97298
Synonyms	serpin peptidase inhibitor, clade F，member 1,Sdf3,Pedfl,Pedf,EPC-1,AI195227
Construction	A DNA sequence encoding the extracellular domain (Met 1-Thr 417) of mouse SerpinF1 (NP_035470.3) precursor was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Gln 20
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	45.8 kDa (predicted); 60-65 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM MES, 150 mM Nacl, 0.02% Tween, 20, 5% Trehalose, pH 5. 5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein that has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression.