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SPOCK3 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 48.3 kDa and the accession number is Q8BKV0-1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μg | $357 | 7-10 days |
Biological Activity | Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-Leu-Arg-AMC. The IC50 value is < 15 nM. |
Description | SPOCK3 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 48.3 kDa and the accession number is Q8BKV0-1. |
Species | Mouse |
Expression System | Baculovirus Insect Cells |
Tag | C-His |
Accession Number | Q8BKV0-1 |
Synonyms | sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3,mKIAA4039,AI428471,2900045C01Rik |
Construction | A DNA sequence encoding the mouse Spock3 (NP_076178.1) (Met1-Ile436) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Ala 23 |
Protein Purity | > 90 % as determined by SDS-PAGE. |
Molecular Weight | 48.3 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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