ST6GAL1 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-02371

ST6GAL1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.9 kDa and the accession number is Q64685.

ST6GAL1 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-02371

ST6GAL1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.9 kDa and the accession number is Q64685.

Pack Size	Price	Availability
100 μg	$451	In Stock
200 μg	$771	7-10 days
500 μg	$1,560	7-10 days

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Product Information

Biological Activity	Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to N-Acetyllactosamine. The specific activity is > 150 pmol/min/μg.
Description	ST6GAL1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.9 kDa and the accession number is Q64685.
Species	Mouse
Expression System	HEK293 Cells
Tag	N-His
Accession Number	Q64685
Synonyms	St6Gal-I,St6galI,St6gal,ST6 β-galactosamide α-2,6-sialyltranferase 1,ST6 beta-galactosamide α-2,6-sialyltranferase 1,ST6 beta-galactosamide alpha-2,6-sialyltranferase 1,Siat1,AW742324
Construction	A DNA sequence encoding the extracellular domain of mouse ST6GAL1 (NP_666045.1) (Lys 27-Cys 403) was fused with a polyhistidine tag at the N-terminus. Predicted N terminal: His
Protein Purity	> 96 % as determined by SDS-PAGE
Molecular Weight	45.9 kDa (predicted); 50-55 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 120 mM NaCl, pH7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein that belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1 / SIAT1 is normally found in the Golgi but can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. β-Galactoside α2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1 / SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.