1.Measured by its binding ability in a functional ELISA. 2. Immobilized mouse ST2-His at 10μg/mL (100μL/well) can bind human TMED1-Fc, the EC50 of human TMED1-Fc is 0.05-0.2μg/mL.

TMED1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 46.2 kDa and the accession number is Q13445.

Human

HEK293 Cells

transmembrane emp24 protein transport domain containing 1,Tp24,IL1RL1LG,Il1rl1l

A DNA sequence encoding the human TMED1 (Q13445) (Met1-Asn194) was expressed, fused with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Ala 24

> 90 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

TMED1 belongs to the EMP24/GP25L family. It contains 1 GOLD domain and is widely expressed. TMED1 binds to its receptor IL1RL1 and results in the activation of DNA binding by nuclear factor NF-kappa-B or transcription from the IL8 promoter and most likely requires other proteins to elicit these activities. Dendritic cells from Peyer's patches (but not from spleen) express TMED1 in response to treatment with LPS. TMED1 may play a role in vesicular protein trafficking, mainly in the early secretory pathway. It may act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side.