6,7-Dimethoxy-4-coumarinylacetic acid (2-(6,7-Dimethoxy-2-oxo-2H-chromen-4-yl)acetic acid) serves as a multifunctional dye with diverse applications in both scientific and traditional domains. This compound facilitates the observation and analysis of cell structures in biological experiments, enabling researchers to track biomolecules, assess cell functions, categorize cell types, identify biomolecules, investigate tissue pathology, and monitor microorganisms. Beyond its scientific utility, 6,7-Dimethoxy-4-coumarinylacetic acid is also employed in industries including textile dyeing, functional textile processing, food pigments, and dye-sensitized solar cells. Its broad utility spans from fundamental research to clinical diagnostics, highlighting its importance across multiple fields.