QHow to dilute working solutions?
ACalculate the dilution factor and volume of the stock solution needed based on the concentration of your working solution. It is recommended to use solvents such as water, saline solution, PBS, or culture medium for dilution. For cell experiments, culture medium is suitable.
Slowly add the appropriate solvent to the stock solution until you reach the desired concentration of your working solution. Gentle vortexing or pipetting can help mix the solution evenly.
Most of our compounds are lipophilic, and precipitation may occur when using PBS or culture medium to dilute the working solution. It is normal and the product can usually be completely dissolved by sonication.
It is recommended to prepare and use the working solution immediately after dilution
QWhat is the calculation method for preparing a solution?
AThe calculation method is as follows:
(1) c=n/v=m/M/v
Concentration=amount of substance/volume=mass/molar mass/volume
(2) The solution preparation table is presented in the the product details page on our website. You can add the volume of solvent as referenced.
QHow should inhibitors be dissolved for cell experiments?
AInhibitors are typically dissolved in DMSO to prepare a concentrated stock solution. Please store at -80°C and aliquot the product to avoid repeated freeze-thaw cycles. For short-term use within one week, aliquots can be stored at 4°C. When diluting in experiments, PBS, physiological saline, culture medium, and water are commonly used. (Note: In cell experiments, the final concentration of DMSO should not exceed 0.1%. If it exceeds 0.1%, it's necessary to conduct a negative control experiment during preliminary test to assess any potential effects of the solvent on cells.)
QCan inhibitors be used for cell experiments?
AYes, our inhibitors can be used for cell/in vitro experiments. However, some compounds may not have literature supporting their use in cell experiments. In such cases, we cannot guarantee efficacy.
QIf the product does not dissolve even when it is below the maximum solubility stated in the instructions, what should I do?
AIt is recommended to utilize some auxiliary dissolution methods, such as heating to 45°C or using sonication to accelerate dissolution.