AC-green (VDP-green), a β-allyl carbamate fluorescent probe, excels at specifically imaging vicinal dithiol proteins (VDPs) within living systems, demonstrating significant sensitivity, particularly in detecting reduced bovine serum albumin (rBSA). With excitation and emission wavelengths of 400 nm and 475 nm respectively (λ ex /λ em =400/475 nm), this probe maintains low toxicity while being highly suitable for sensing VDPs in living cells and zebrafish [1].

AC-green (VDP-green) exhibits over a 60-fold increase in emission in aqueous solution in response to VDPs, without significant interference from biological thiols, amino acids, or inorganic salts [1]. At 2 μM concentration and 90 minutes, AC-green shows no fluorescence signal within the pH range of 5.0-9.0 until rBSA is added, which activates fluorescence [1]. At 10 μM, AC-green has low cytotoxicity in HepG2 and HeLa cells and can image VDPs in living HepG2 cells within 15 minutes, producing bright green fluorescence, which is inhibited by PAO, a specific ligand for protein vicinal dithiols [1]. Additionally, a 10 μM concentration of AC-green incubated for 20 minutes with zebrafish generates a strong fluorescence signal in the green channel [1].