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Anti-APOE Monoclonal Antibody-AF488
Catalog No. TMAZ-0071A4
Alias Apolipoprotein E
Anti-APOE Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting APOE. Anti-APOE Monoclonal Antibody-AF488 can be used in ELISA, FACS.
Anti-APOE Monoclonal Antibody-AF488
Anti-APOE Monoclonal Antibody-AF488
Catalog No. TMAZ-0071A4Alias Apolipoprotein E
Anti-APOE Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting APOE. Anti-APOE Monoclonal Antibody-AF488 can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $570 | 12 days | |
| 50 μg | $945 | 12 days | |
| 100 μg | $1,415 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-APOE Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting APOE. Anti-APOE Monoclonal Antibody-AF488 can be used in ELISA, FACS. |
| Alias | Apolipoprotein E |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of APOE overexpressed 461F cells with TMAZ-0071A4, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of APOE protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids (PubMed: 14754908, PubMed: 1911868, PubMed: 6860692). APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance (PubMed: 14754908, PubMed: 1911868, PubMed: 1917954, PubMed: 23620513, PubMed: 2762297, PubMed: 6860692, PubMed: 9395455). Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma (PubMed: 2762297, PubMed: 6860692, PubMed: 9395455). As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL) (PubMed: 1911868, PubMed: 6860692). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles (PubMed: 12950167, PubMed: 1530612, PubMed: 1917954, PubMed: 20030366, PubMed: 20303980, PubMed: 2063194, PubMed: 2762297, PubMed: 7635945, PubMed: 7768901, PubMed: 8756331, PubMed: 8939961). Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells (PubMed: 23676495, PubMed: 7635945, PubMed: 9395455, PubMed: 9488694). A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes (PubMed: 1911868, PubMed: 1917954, PubMed: 23676495, PubMed: 29516132, PubMed: 9395455). APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues (PubMed: 2762297, PubMed: 29516132). By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis (PubMed: 1917954, PubMed: 2762297, PubMed: 29516132). APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis (PubMed: 14754908, PubMed: 23620513, PubMed: 9395455). First, it is functionally associated with ABCA1 in the biogenesis of HDLs in tissues (PubMed: 14754908, PubMed: 23620513). Second, it is enriched in circulating HDLs and mediates their uptake by hepatocytes (PubMed: 9395455). APOE also plays an important role in lipid transport in the central nervous system, regulating neuron survival and sprouting (PubMed: 25173806, PubMed: 8939961). APOE is also involved in innate and adaptive immune responses, controlling for instance the survival of myeloid-derived suppressor cells (By similarity). Binds to the immune cell receptor LILRB4 (PubMed: 30333625). APOE may also play a role in transcription regulation through a receptor-dependent and cholesterol-independent mechanism, that activates MAP3K12 and a non-canonical MAPK signal transduction pathway that results in enhanced AP-1-mediated transcription of APP (PubMed: 28111074)|(Microbial infection) Through its interaction with HCV envelope glycoprotein E2, participates in the attachment of HCV to HSPGs and other receptors (LDLr, VLDLr, and SR-B1) on the cell surface and to the assembly, maturation and infectivity of HCV viral particles (PubMed: 25122793, PubMed: 29695434). This interaction is probably promoted via the up-regulation of cellular autophagy by the virus (PubMed: 29695434) |
| Conjucates | AF488 |
| Others Formats | Biotin/APC/PE/AF555/AF647 |
| Antibody Types Available | 5 |
| Immunogen | APOE |
| Antigen Species | Human |
| Gene ID | 348 |
| Uniprot ID | |
| Biology Area | Immunology Research |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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