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Anti-CHGA Monoclonal Antibody-AF555
Catalog No. TMAZ-0139A5
Alias Pituitary secretory protein I, Chromogranin-A
Anti-CHGA Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting CHGA. Anti-CHGA Monoclonal Antibody-AF555 can be used in ELISA, FACS.
Anti-CHGA Monoclonal Antibody-AF555
Anti-CHGA Monoclonal Antibody-AF555
Catalog No. TMAZ-0139A5Alias Pituitary secretory protein I, Chromogranin-A
Anti-CHGA Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting CHGA. Anti-CHGA Monoclonal Antibody-AF555 can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $385 | 12 days | |
| 50 μg | $640 | 12 days | |
| 100 μg | $960 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CHGA Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting CHGA. Anti-CHGA Monoclonal Antibody-AF555 can be used in ELISA, FACS. |
| Alias | Pituitary secretory protein I, Chromogranin-A |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of CHGA overexpressed 486F cells with TMAZ-0139A5, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Single-pass type I membrane |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of CHGA protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Strongly inhibits glucose induced insulin release from the pancreas|Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed: 15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed: 15723172, PubMed: 24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed: 21214543). Acts as a potent scavenger of free radicals in vitro (PubMed: 24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed: 18541522)|Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation |
| Conjucates | AF555 |
| Others Formats | Biotin/APC/PE/AF488/AF647 |
| Antibody Types Available | 1 |
| Immunogen | CHGA |
| Antigen Species | Human |
| Gene ID | 1113 |
| Uniprot ID | |
| Biology Area | Cancer Research |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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