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Anti-CHGA Monoclonal Antibody-AF647

Catalog No. TMAZ-0139A6
Alias Pituitary secretory protein I, Chromogranin-A

Anti-CHGA Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting CHGA. Anti-CHGA Monoclonal Antibody-AF647 can be used in ELISA, FACS.

Anti-CHGA Monoclonal Antibody-AF647

Anti-CHGA Monoclonal Antibody-AF647

Catalog No. TMAZ-0139A6Alias Pituitary secretory protein I, Chromogranin-A
Anti-CHGA Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting CHGA. Anti-CHGA Monoclonal Antibody-AF647 can be used in ELISA, FACS.
Pack SizePriceAvailabilityQuantity
25 μg$38512 days
50 μg$64012 days
100 μg$96012 days
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Product Introduction

Bioactivity
Description
Anti-CHGA Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting CHGA. Anti-CHGA Monoclonal Antibody-AF647 can be used in ELISA, FACS.
AliasPituitary secretory protein I, Chromogranin-A
Ig Type
Rabbit monoclonal IgG
Reactivity
Human
Verified Activity
Flow cytometry analysis of CHGA overexpressed 486F cells with TMAZ-0139A6, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line).
Application
ELISA, FACS
Recommended Dose
0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationSingle-pass type I membrane
ConstructionThis antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of CHGA protein.
PurificationProtein A Affinity Purified
AppearanceLiquid
Formulation0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin.
Purity> 95% as determined by SDS-PAGE.
Research BackgroundStrongly inhibits glucose induced insulin release from the pancreas|Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed: 15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed: 15723172, PubMed: 24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed: 21214543). Acts as a potent scavenger of free radicals in vitro (PubMed: 24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed: 18541522)|Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation
Related Conjugates and Formulations
Conjucates
AF647
Others Formats
Biotin/APC/PE/AF488/AF555
Antibody Types Available
1
Antigen Details
Immunogen
CHGA
Antigen Species
Human
Gene ID
1113
Uniprot ID
Biology Area
Cancer Research
Chemical Properties
Molecular Weight150 kDa
Stability & Storage
Stability & StorageFor unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze.
TransportFor unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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