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Anti-KITLG Monoclonal Antibody-AF555
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Catalog No. TMAZ-0211A5
Alias Stem cell factor, Mast cell growth factor, Kit ligand, c-Kit ligand
Anti-KITLG Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting KITLG. Anti-KITLG Monoclonal Antibody-AF555 can be used in ELISA, FACS, IF.
Anti-KITLG Monoclonal Antibody-AF555
Anti-KITLG Monoclonal Antibody-AF555
😃Good
Catalog No. TMAZ-0211A5Alias Stem cell factor, Mast cell growth factor, Kit ligand, c-Kit ligand
Anti-KITLG Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting KITLG. Anti-KITLG Monoclonal Antibody-AF555 can be used in ELISA, FACS, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $230 | 12 days | |
| 50 μg | $385 | 12 days | |
| 100 μg | $575 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-KITLG Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting KITLG. Anti-KITLG Monoclonal Antibody-AF555 can be used in ELISA, FACS, IF. |
| Alias | Stem cell factor, Mast cell growth factor, Kit ligand, c-Kit ligand |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of KITLG overexpressed 427F cells with TMAZ-0211A5, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS, IF |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Single-pass type I membrane |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of KITLG protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins |
| Conjucates | AF555 |
| Others Formats | Biotin/APC/PE/AF488/AF647 |
| Antibody Types Available | 2 |
| Immunogen | KITLG |
| Antigen Species | Human |
| Gene ID | 4254 |
| Uniprot ID | |
| Biology Area | Cytokines |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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