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Anti-PDPN Monoclonal Antibody-AF488
Catalog No. TMAZ-0063A4
Alias T1-alpha, Podoplanin, PA2.26 antigen, Glycoprotein 36, Aggrus
Anti-PDPN Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting PDPN. Anti-PDPN Monoclonal Antibody-AF488 can be used in ELISA, FACS.
Anti-PDPN Monoclonal Antibody-AF488
Anti-PDPN Monoclonal Antibody-AF488
Catalog No. TMAZ-0063A4Alias T1-alpha, Podoplanin, PA2.26 antigen, Glycoprotein 36, Aggrus
Anti-PDPN Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting PDPN. Anti-PDPN Monoclonal Antibody-AF488 can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $175 | 12 days | |
| 50 μg | $290 | 12 days | |
| 100 μg | $435 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PDPN Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting PDPN. Anti-PDPN Monoclonal Antibody-AF488 can be used in ELISA, FACS. |
| Alias | T1-alpha, Podoplanin, PA2.26 antigen, Glycoprotein 36, Aggrus |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of PDPN overexpressed 470F cells with TMAZ-0063A4, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of PDPN protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation (PubMed: 14522983, PubMed: 15231832, PubMed: 17222411, PubMed: 17616532, PubMed: 18215137). Interaction with CD9, on the contrary, attenuates platelet aggregation induced by PDPN (PubMed: 18541721). Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness (PubMed: 17046996, PubMed: 21376833). Interaction with CD44 promotes directional cell migration in epithelial and tumor cells (PubMed: 20962267). In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR; MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR; MSN and RDX) and MYL9 activation (By similarity). Through binding with LGALS8 may participate in connection of the lymphatic endothelium to the surrounding extracellular matrix (PubMed: 19268462). In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion (PubMed: 15515019). Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1 (PubMed: 25486435). Required for normal lung cell proliferation and alveolus formation at birth (By similarity). Does not function as a water channel or as a regulator of aquaporin-type water channels (PubMed: 9651190). Does not have any effect on folic acid or amino acid transport (By similarity) |
| Conjucates | AF488 |
| Others Formats | Biotin/APC/PE/AF555/AF647 |
| Antibody Types Available | 1 |
| Immunogen | PDPN |
| Antigen Species | Human |
| Gene ID | 10630 |
| Uniprot ID | |
| Biology Area | Immunology Research |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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