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Anti-SEMA4D Monoclonal Antibody-AF488
Catalog No. TMAZ-0130A4
Alias Semaphorin-4D, GR3, BB18, A8
Anti-SEMA4D Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting SEMA4D. Anti-SEMA4D Monoclonal Antibody-AF488 can be used in ELISA, FACS.
Anti-SEMA4D Monoclonal Antibody-AF488
Anti-SEMA4D Monoclonal Antibody-AF488
Catalog No. TMAZ-0130A4Alias Semaphorin-4D, GR3, BB18, A8
Anti-SEMA4D Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting SEMA4D. Anti-SEMA4D Monoclonal Antibody-AF488 can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $300 | 12 days | |
| 50 μg | $500 | 12 days | |
| 100 μg | $745 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-SEMA4D Monoclonal Antibody-AF488 is a Biotin-conjugated rabbit antibody targeting SEMA4D. Anti-SEMA4D Monoclonal Antibody-AF488 can be used in ELISA, FACS. |
| Alias | Semaphorin-4D, GR3, BB18, A8 |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of SEMA4D overexpressed 293F cells with TMAZ-0130A4BI, followed by goat anti-rabbit IgG-ABflo 647 (red). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Single-pass type I membrane |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of SEMA4D protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed: 20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed: 19788569). Promotes the migration of cerebellar granule cells (PubMed: 16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed: 8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed: 16055703) |
| Conjucates | AF488 |
| Others Formats | Biotin/APC/PE/AF555/AF647 |
| Antibody Types Available | 3 |
| Immunogen | SEMA4D |
| Antigen Species | Human |
| Gene ID | 10507 |
| Uniprot ID | |
| Biology Area | Cancer Research |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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