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Anti-SEMA4D Monoclonal Antibody-PE
Anti-SEMA4D Monoclonal Antibody-PE
Anti-SEMA4D Monoclonal Antibody-PE is a Biotin-conjugated rabbit antibody targeting SEMA4D. Anti-SEMA4D Monoclonal Antibody-PE can be used in ELISA, FACS.
Catalog No. TMAZ-0130P
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Anti-SEMA4D Monoclonal Antibody-PE
Catalog No. TMAZ-0130PAlias Semaphorin-4D, GR3, BB18, A8
Anti-SEMA4D Monoclonal Antibody-PE is a Biotin-conjugated rabbit antibody targeting SEMA4D. Anti-SEMA4D Monoclonal Antibody-PE can be used in ELISA, FACS.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose. | Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $300 | 12 days | |
| 50 μg | $500 | 12 days | |
| 100 μg | $745 | 12 days |
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Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-SEMA4D Monoclonal Antibody-PE is a Biotin-conjugated rabbit antibody targeting SEMA4D. Anti-SEMA4D Monoclonal Antibody-PE can be used in ELISA, FACS. |
| Alias | Semaphorin-4D, GR3, BB18, A8 |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of SEMA4D overexpressed 293F cells with TMAZ-0130PBI, followed by goat anti-rabbit IgG-ABflo 647 (red). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Single-pass type I membrane |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of SEMA4D protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed: 20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed: 19788569). Promotes the migration of cerebellar granule cells (PubMed: 16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed: 8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed: 16055703) |
| Conjucates | PE |
| Others Formats | Biotin/APC/AF488/AF555/AF647 |
| Antibody Types Available | 3 |
| Immunogen | SEMA4D |
| Antigen Species | Human |
| Gene ID | 10507 |
| Uniprot ID | |
| Biology Area | Cancer Research |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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