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Anti-SEMA4D Monoclonal Antibody-PE

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Catalog No. TMAZ-0130P
Alias Semaphorin-4D, GR3, BB18, A8

Anti-SEMA4D Monoclonal Antibody-PE is a Biotin-conjugated rabbit antibody targeting SEMA4D. Anti-SEMA4D Monoclonal Antibody-PE can be used in ELISA, FACS.

Anti-SEMA4D Monoclonal Antibody-PE

Anti-SEMA4D Monoclonal Antibody-PE

😃Good
Catalog No. TMAZ-0130PAlias Semaphorin-4D, GR3, BB18, A8
Anti-SEMA4D Monoclonal Antibody-PE is a Biotin-conjugated rabbit antibody targeting SEMA4D. Anti-SEMA4D Monoclonal Antibody-PE can be used in ELISA, FACS.
Pack SizePriceAvailabilityQuantity
25 μg285 €12 days
50 μg475 €12 days
100 μg707 €12 days
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Product Introduction

Bioactivity
Description
Anti-SEMA4D Monoclonal Antibody-PE is a Biotin-conjugated rabbit antibody targeting SEMA4D. Anti-SEMA4D Monoclonal Antibody-PE can be used in ELISA, FACS.
AliasSemaphorin-4D, GR3, BB18, A8
Ig Type
Rabbit monoclonal IgG
Reactivity
Human
Verified Activity
Flow cytometry analysis of SEMA4D overexpressed 293F cells with TMAZ-0130PBI, followed by goat anti-rabbit IgG-ABflo 647 (red). The isotype control is rabbit IgG (black line).
Application
ELISA, FACS
Recommended Dose
0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationSingle-pass type I membrane
ConstructionThis antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of SEMA4D protein.
PurificationProtein A Affinity Purified
AppearanceLiquid
Formulation0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin.
Purity> 95% as determined by SDS-PAGE.
Research BackgroundCell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed: 20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed: 19788569). Promotes the migration of cerebellar granule cells (PubMed: 16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed: 8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed: 16055703)
Related Conjugates and Formulations
Conjucates
PE
Others Formats
Biotin/APC/AF488/AF555/AF647
Antibody Types Available
3
Antigen Details
Immunogen
SEMA4D
Antigen Species
Human
Gene ID
10507
Uniprot ID
Biology Area
Cancer Research
Chemical Properties
Molecular Weight150 kDa
Stability & Storage
Stability & StorageFor unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze.
TransportFor unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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