CSF1R-IN-21 (compound 7e), a CSF-1R inhibitor, exhibits potent inhibition with an IC 50 of 31 nM and effectively blocks CSF-1R auto-phosphorylation. It is utilized in researching neurodegenerative diseases [1].

CSF-1R:31 nM

CSF1R-IN-21 (compound 7e) at a concentration of 10 μM for 6 hours inhibits CSF-1R autophosphorylation and exhibits minimal cytotoxicity in the EOC 20 cell line according to cell viability assays [1]. At 50 μM for 4 hours, it shows good blood-brain barrier permeability in parallel artificial membrane permeability assays (PAMPA) [1]. The compound demonstrates good performance in plasma stability tests at 10 µM (0/30/120 min) [1]. In microsomal stability tests, CSF1R-IN-21 exhibits moderate stability at a concentration of 1 mM for 30 minutes [1]. In cell viability assays, the compound showed very low cytotoxic effects on the microglial cell line EOC 20 [1].