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Janelia Fluor® 585, SE

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Catalog No. T39243Cas No. 1811539-88-4
Alias JF585,NHS, JF585, SE

Janelia Fluor 585, SE (JF585, SE) is an orange fluorescent dye with an NHS ester that can conjugate with primary amine groups, making it immediately useful for structured illumination (SIM) and stimulated emission depletion (STED) imaging, and convertible to a photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments.

Janelia Fluor® 585, SE

Janelia Fluor® 585, SE

😃Good
Catalog No. T39243Alias JF585,NHS, JF585, SECas No. 1811539-88-4
Janelia Fluor 585, SE (JF585, SE) is an orange fluorescent dye with an NHS ester that can conjugate with primary amine groups, making it immediately useful for structured illumination (SIM) and stimulated emission depletion (STED) imaging, and convertible to a photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments.
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Product Introduction

Bioactivity
Description
Janelia Fluor 585, SE (JF585, SE) is an orange fluorescent dye with an NHS ester that can conjugate with primary amine groups, making it immediately useful for structured illumination (SIM) and stimulated emission depletion (STED) imaging, and convertible to a photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments.
In vitro
The chemical compound exhibits a maximum absorption wavelength (λabs) of 585 nm and a maximum emission wavelength (λem) of 609 nm[1]. The JF585-SNAP-tag ligand (24) serves effectively as a cellular label. Furthermore, the novel HaloTag ligands, developed from JF585 and JF635, display significant chromogenicity and fluorogenicity, essential for advanced imaging experiments[1].
AliasJF585,NHS, JF585, SE
Chemical Properties
Molecular Weight649.599
FormulaC34H27F4N3O6
Cas No.1811539-88-4
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

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Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
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Janelia Fluor® 585, SE
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