QWhether sonication is needed for product dissolution? What if I don't have an ultrasound machine?
Asonication can accelerate dissolution. If the compound is not dissolving, sonication is recommended. If you don't have an ultrasound machine, a lower solvent concentration or using less powder are recommended.
QHow to choose the appropriate solvent?
ACommon solvents include DMSO, water, ethanol, etc. Please select the appropriate solvent according to the solubility reference. If the inhibitor can be dissolved in DMSO, it is recommended to use freshly opened DMSO without moisture absorption. If the inhibitor can be dissolved in water, solvents like sterile water, physiological saline, sterile PBS ahd culture medium are recommended.
QWhat's the meaning of "< 1 mg/mL refers to the slightly soluble or insoluble"?
AIt means that if the solubility is less than 1 mg/mL
QDoes the product need to be protected from light?
AGenerally, for molecules that require light-protected storage, we will ship them in amber glass bottles.
QCan the inhibitor DMSO stock solution be directly diluted with buffer to create a gradient?
AIn most cases, it can dissolve. However, sometimes organic reagents may precipitate when directly added to an aqueous medium. It is recommended to first dilute the inhibitor with DMSO to form a gradient, and then add the diluted inhibitor to the buffer or cell culture medium. Some inhibitors may only dissolve in the aqueous phase at their working concentrations.
For example, if a final concentration of 1 μM is desired in cell experiments, the 10 mM DMSO stock solution can be diluted to 1 mM with DMSO, and then 2 μL can be drawn and added to 2 mL of saline/PBS/cell culture medium, resulting in a final concentration of 1 μM.
To avoid precipitation of the drug, it is recommended to preheat the mother liquor and culture medium to 37°C before dilution to avoid serious precipitation caused by low temperature. If precipitation occurs during the dilution process, it is suggested to use ultrasonic heating to redissolve the compound.