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NFQ1

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Catalog No. T87001Cas No. 1262391-07-0

NFQ1, a non-fluorescent quencher (absorption wavelength: 474 nm), is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method, enabling measurement of unquenched donor emission in the same sample. NFQ1 shows a broad absorption spectrum, ensuring its utility as a dark acceptor for many donors [1].

NFQ1

NFQ1

😃Good
Catalog No. T87001Cas No. 1262391-07-0
NFQ1, a non-fluorescent quencher (absorption wavelength: 474 nm), is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method, enabling measurement of unquenched donor emission in the same sample. NFQ1 shows a broad absorption spectrum, ensuring its utility as a dark acceptor for many donors [1].
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Product Introduction

Bioactivity
Description
NFQ1, a non-fluorescent quencher (absorption wavelength: 474 nm), is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method, enabling measurement of unquenched donor emission in the same sample. NFQ1 shows a broad absorption spectrum, ensuring its utility as a dark acceptor for many donors [1].
In vitro
NFQ1 is used for the One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method, employing a novel, non-fluorescent methanethiosulfonate-linked acceptor that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond. After quantitating the quenched donor emission, the acceptor is removed by reduction, enabling measurement of unquenched donor emission in the same sample [1].
Chemical Properties
Molecular Weight451.48
FormulaC17H17N5O6S2
Cas No.1262391-07-0
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

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TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
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