Tubulin inhibitor 16 is a potent tubulin inhibitor that exhibits antiproliferative activity and cytotoxicity in HepG2 cells [1].

Tubulin inhibitor 16 (compound 2) (0-25 μM, 24 h followed by a 72 h compound-free incubation period) shows antiproliferative activity with IC50 values of 1.43, 0.19, 2.33, 0.58, 0.83, 0.20, >25 μM for MCF7, MDA-MB-231, HepG2, SNU423, A549, HCT116, and THLE-3 cells [1]. Cell Proliferation Assay [1] reveals IC50 values of 0.75, 0.17, 3.12, 0.41, 0.37, 0.22 μM for MCF7, MDA-MB-231, HepG2, SNU423, A549, HCT116 cells, respectively, with a 0-50 μM concentration over 96 h incubation time. In the Cell Cytotoxicity Assay [1], no colonies were evident when HepG2 cells were treated with 0.5 or 1 μM for 14 days.