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14-3-3 sigma Protein, Human, Recombinant (GST)

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14-3-3 sigma Protein, Human, Recombinant (GST)

Catalog No. TMPY-01263

14-3-3 sigma Protein, Human, Recombinant (GST) is expressed in E. coli expression system with GST tag. The predicted molecular weight is 50.1 kDa and the accession number is P31947-1.

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Pack Size	Price	Availability	Quantity
100 μg	$600	7-10 days

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Biological Description

Biological Information	Testing in progress
Description	14-3-3 sigma Protein, Human, Recombinant (GST) is expressed in E. coli expression system with GST tag. The predicted molecular weight is 50.1 kDa and the accession number is P31947-1.
Species	Human
Expression System	E. coli
Tag	N-GST
Accession Number	P31947-1
Synonyms	14-3-3 σ,stratifin,YWHAS
Construction	The Human SFN (NP_006133.1) (Met 1-Ser 248) was fused with the GST tag at the N-terminus.
Protein Purity	> 94 % as determined by SDS-PAGE
Molecular Weight	50.1 kDa (predicted)
Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile 20 mM Tris, 150 mM NaCl, 10 mM GSH, 25% glycerol, pH 8.0.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the 14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathways. More than 1 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.