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Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $462 | 7-10 days | |
100 μg | $1,070 | 7-10 days |
Biological Information | 1. Immobilized 4-1BB-Fch at 10 μg/mL (100 μL/well) can bind S3-Avi-Fc-TNFSF9 , the EC50 of S3-Avi-Fc-TNFSF9 is 7.0-20 ng/mL.
2. Measured by it's ability to induce IL-8 secretion by HT1080 transfected with human CD137. The ED50 for this effect is typically 0.25-1.5 μg/mL. |
Description | 4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (Avi & Fc), Biotinylated is expressed in HEK293 mammalian cells with AVI and Fc tag. The predicted molecular weight is 47.98 kDa and the accession number is A0A0U5J8I0. |
Species | Human |
Expression System | HEK293 Cells |
Tag | N-Avi-hFc |
Accession Number | A0A0U5J8I0 |
Synonyms | 4-1BBL,4-1BB-L,CD137L,tumor necrosis factor (ligand) superfamily, member 9 |
Construction | The Human TNFSF9 (NP_003802.1) (Arg71-Glu254) was expressed with a N-terminal AVI tag followed by a Fc region of Human IgG1 tag. The purified protein was biotinylated in vitro. |
Protein Purity | > 95 % as determined by SDS-PAGE. |
Molecular Weight | 47.98 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | 4-1BB ligand is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In addition, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
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