ANGPTL3 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-00285

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake. ANGPTL3 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 26.6 kDa and the accession number is Q9Y5C1.

ANGPTL3 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-00285

Pack Size	Price	Availability
100 μg	$814	7-10 days
500 μg	$3,250	7-10 days
1 mg	$5,440	7-10 days

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Questions

QWhat should I do if the IC50 obtained for cell killing are significantly different from that presented on the your website?

AThe IC50 you obtain refers to the cell proliferation inhibition assay (half maximal inhibitory concentration), which is different in meaning from the IC50 on our website. The IC50 often refers to the inhibition rate of the target without cell experiments, which is generally determined through kinase or protein purification experiments, and is commonly reported in nanomolar concentrations. However, the IC50 obtained from cell proliferation inhibition assays refers to the half-maximal lethality of the cells, which involves cellular metabolism and penetration, so the concentration is usually higher. Furthermore, the effect of the same compound on different cell models varies. It is recommended to try increasing the incubation volume and extending the incubation time.

QWhat do "mg/mL" and "mM" mean?

Amg/mL" and "mM" are two different units of measurement, but represent the same maximum solubility. You can convert between them using the molar concentration calculator on our website.

QHow to dilute working solutions?

ACalculate the dilution factor and volume of the stock solution needed based on the concentration of your working solution. It is recommended to use solvents such as water, saline solution, PBS, or culture medium for dilution. For cell experiments, culture medium is suitable. Slowly add the appropriate solvent to the stock solution until you reach the desired concentration of your working solution. Gentle vortexing or pipetting can help mix the solution evenly. Most of our compounds are lipophilic, and precipitation may occur when using PBS or culture medium to dilute the working solution. It is normal and the product can usually be completely dissolved by sonication. It is recommended to prepare and use the working solution immediately after dilution

QWhat should I do if the products in powder form adhere to the vial tightly?

AIt's recommended to centrifuge it at 3000 rpm for a few minutes.

QAnimals cannot tolerate DMSO well. How should the dosage of DMSO be controlled during administration?

AFor normal mice, the concentration of DMSO should be kept below 10%. For nude mice, transgenic mice, or mice with weak tolerance, the concentration of DMSO should be kept below 2%. For inhibitors being used for the first time, it is recommended to perform a solvent-negative control experiment to confirm that the solvent has no nonspecific effects on the animals.

Biological Activity	Immobilized Anti-ANGPTL3 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ANGPTL3, His Tag with the EC50 of 5.0ng/ml determined by ELISA.
Description	ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake. ANGPTL3 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 26.6 kDa and the accession number is Q9Y5C1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	Q9Y5C1
Synonyms	FHBL2,ANL3,ANGPTL3,ANGPT5,Angiopoietin-like Protein 3,Angiopoietin-5,ANG-5
Construction	Ser17-Lys219
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	26.6 kDa (predicted). Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.

ANGPTL3 Protein, Human, Recombinant (His & Avi), Biotinylated

ANGPTL3 Protein, Human, Recombinant (His & Avi), Biotinylated

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