Apolipoprotein H/APOH Protein, Cynomolgus, Recombinant (His)

Catalog No. TMPK-01328

Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH*1, APOH*2, APOH*3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH*2 is the most frequent allele.

Apolipoprotein H/APOH Protein, Cynomolgus, Recombinant (His)

Catalog No. TMPK-01328

Pack Size	Price	Availability
100 μg	$487	7-10 days
500 μg	$1,950	7-10 days
1 mg	$3,250	7-10 days

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Product Information

Biological Activity	Immobilized Cynomolgus APOH, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 64.7ng/ml determined by ELISA.
Description	Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH1, APOH2, APOH3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH2 is the most frequent allele.
Species	Cynomolgus
Expression System	HEK293 Cells
Tag	C-His
Accession Number	XP_045230479.1
Synonyms	β(2)GPI,BG,beta(2)GPI,B2GPI,B2GP1,B2G1,Apo-H,APOH
Construction	Gly20-Cys345
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	37.39 kDa (predicted). Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 100 mM Arginine (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH1, APOH2, APOH3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH2 is the most frequent allele.