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B4GALT1 Protein, Mouse, Recombinant (His)

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B4GALT1 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-06809

B4GALT1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 41.12 kDa and the accession number is P15535.

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Pack Size	Price	Availability	Quantity
100 μg	$1,210	7-10 days
1 mg	$7,860	7-10 days

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Biological Description

Biological Information	Testing in progress
Description	B4GALT1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 41.12 kDa and the accession number is P15535.
Species	Mouse
Expression System	HEK293 Cells
Tag	N-His
Accession Number	P15535
Synonyms	Ggtb,β4Gal-T1,B-1,4-GalT,GalT,β-1,4-GalT1,UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1,B-1,4-GalT1,b4Gal-T1,beta-1,4-GalT,β-1,4-GalT,UDP-Gal:βGlcNAc β 1,4- galactosyltransferase, polypeptide 1,beta-1,4-GalT1,Ggtb2,beta4Gal-T1
Construction	The Mouse B4GALT1 (P15535) (Gly44-Arg399) was expressed, with a polyhistidine tag at the N-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Molecular Weight	41.12 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.