B7-H3 Protein, Human, Recombinant (aa 29-245, hFc)

Catalog No. TMPK-01017

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. B7-H3 Protein, Human, Recombinant (aa 29-245, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 50.1 kDa and the accession number is Q5ZPR3-2.

B7-H3 Protein, Human, Recombinant (aa 29-245, hFc)

Catalog No. TMPK-01017

Pack Size	Price	Availability
100 μg	352 €	7-10 days
500 μg	1.415 €	7-10 days
1 mg	2.356 €	7-10 days

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Product Information

Biological Activity	Immobilized Human B7-H3, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 62.9ng/ml determined by ELISA.
Description	B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. B7-H3 Protein, Human, Recombinant (aa 29-245, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 50.1 kDa and the accession number is Q5ZPR3-2.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	Q5ZPR3-2
Synonyms	PSEC0249,PRO352,CD276,B7-H3,B7H3,B7 homolog 3, UNQ309
Construction	Leu29-Pro245
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	50.1 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.