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B7-H5/Gi24/VISTA Protein, Human, Recombinant (hFc)

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B7-H5/Gi24/VISTA Protein, Human, Recombinant (hFc)

Catalog No. TMPK-01185

B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. B7-H5/Gi24/VISTA Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 44.9 kDa and the accession number is Q9H7M9.

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Pack Size	Price	Availability
100 μg	$324	7-10 days
500 μg	$1,300	7-10 days
1 mg	$2,170	7-10 days

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Biological Description

Biological Information	1. Immobilized Human B7-H5, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). 2. Human B7-H5, hFc Tag captured on CM5 Chip via Protein A can bind Human IGSF11, His Tag with an affinity constant of 14.44 μM as determined in SPR assay.
Description	B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. B7-H5/Gi24/VISTA Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 44.9 kDa and the accession number is Q9H7M9.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	Q9H7M9
Synonyms	PP2135,SISP1,VISTA,Gi24,B7H5,4632428N05Rik,Dies1,PD1H,VSIR,B7-H5,C10orf54,PD-1H
Construction	Phe33-Ala194
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	44.9 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.