BACE1 Protein, Human, Recombinant (hFc)

Catalog No. TMPY-00651

BACE1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 75 kDa and the accession number is A0A024R3D7.

BACE1 Protein, Human, Recombinant (hFc)

Catalog No. TMPY-00651

BACE1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 75 kDa and the accession number is A0A024R3D7.

Pack Size	Price	Availability	Quantity
100 μg	$600	7-10 days
1 mg	$3,910	7-10 days

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Product Information

Biological Activity	Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >0.5 pmoles/min/μg.
Description	BACE1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 75 kDa and the accession number is A0A024R3D7.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P56817
Synonyms	β-site APP-cleaving enzyme 1,HSPC104,beta-site APP-cleaving enzyme 1,BACE,ASP2
Construction	A DNA sequence encoding the extracellular domain (Met 1-Thr 457) of human BACE1 (NP_036236.1) was expressed as a secreted chimeric protein with the C-terminal fused Fc region of human IgG1. Predicted N terminal: Thr 22
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	75 kDa (predicted); 100-105 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.