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Bat coronavirus 133/2005 Nucleoprotein/NP Protein (His)

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Bat coronavirus 133/2005 Nucleoprotein/NP Protein (His)

Catalog No. TMPH-00192

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Bat coronavirus 133/2005 Nucleoprotein/NP Protein (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 51.9 kDa and the accession number is Q0Q4E6.

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Pack Size	Price	Availability
20 μg	$360	20 days
100 μg	$678	20 days
1 mg	$2,300	20 days

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Biological Description

Description	Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Bat coronavirus 133/2005 Nucleoprotein/NP Protein (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 51.9 kDa and the accession number is Q0Q4E6.
Species	BtCoV
Expression System	E. coli
Tag	N-6xHis
Accession Number	Q0Q4E6
Synonyms	Nucleocapsid protein,Protein N,NC,Nucleoprotein
Amino Acid	MATPAAPRTISFADNNDNQPNQQQRGRGRNPKPRPAPNNTVSWYTGLTQHGKNPLAFPPGQGVPLNANSTTAQNAGYWRRQDRKINTGNGVKQLAPRWFFYYTGTGPEANLPFRSVKDGIVWVYEEGATDAPSVFGTRNPANDAAIVCQFAPGTLIPKNFHIEGTGGNSQSSSRASSNSRNSSRSNSRGGRSTSNSRGTSPVSHGVGSAESLAALPLLLDLQKRLADLESGKSKQPKVVTKKDAAAAKNKMRHKRVATKGFNVTQAFGLRGPGPLQGNFGDMNYNKFGTEDPRWPQMAELAPSASAFMSMSQFKLTHQSNDDKGDPIYFLSYSGAIKLDPKNPNYKKWLELLEANIDAYKTFPKKERKPKTTEDGAVVASSSASQMEDVDAKPQRKPKSRVAGSITMRSGSSPALQDVTFDSEA
Construction	1-424 aa
Protein Purity	> 90% as determined by SDS-PAGE.
Molecular Weight	51.9 kDa (predicted)
Formulation	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

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