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Caspase-3 Protein, Human, Recombinant (aa 29-277, His) is expressed in E. coli. The accession number is P42574.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $687 | Backorder | |
1 mg | $4,560 | Backorder |
Biological Activity | Measured by its ability to cleave the fluorogenic peptide substrate Ac-DEVD-AFC. The specific activity is > 5000 pmoles/min/μg. |
Description | Caspase-3 Protein, Human, Recombinant (aa 29-277, His) is expressed in E. coli. The accession number is P42574. |
Species | Human |
Expression System | E. coli |
Tag | C-His |
Accession Number | P42574 |
Synonyms | Yama,SCA-1,CPP32B,CPP32,apopain |
Construction | A DNA sequence encoding the mature form of human caspase3 (P42574) (Ser29-His277) was expressed with a polyhistidine tag at the C-terminus. |
Protein Purity | > 95% as determined by SDS-PAGE |
Molecular Weight | 29.9 kDa (predicted); 17 and 15 kDa (reducing contition) |
Endotoxin | Please contact us for more information. |
Formulation | Lyophilized from sterile 20mM HEPES, 100mM NaCl, 1mM EDTA, 10% Sucrose, 0.1% chaps, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
Reconstitution | Please refer to the lot-specific COA. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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