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Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $801 | 7-10 days | |
1 mg | $5,220 | 7-10 days |
Biological Information | 1. Loaded CD122 / IL2RB Protein, Human, Recombinant (Fc Tag) on Pro A Biosensor, can bind IL2, Human, Mutant with an affinity constant of was 0.3 μM as determined by Octet RED System.
2. Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED50 for this effect is 1.0-3.8 μg/mL. |
Description | CD122/IL2RB Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 51.3 kDa and the accession number is P14784. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-hFc |
Accession Number | P14784 |
Synonyms | IL-15RB,interleukin 2 receptor, beta,interleukin 2 receptor, β,IL15RB,CD122,P70-75 |
Construction | The extracellular domain (Met1-Asp239) of Human IL2RB (NP_000869.1) was expressed with the C-terminal Fc region of Human IgG1. |
Protein Purity | ≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
Molecular Weight | 51.3 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Interleukin-2 receptor (IL-2R) also known as High-affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms concerning the ability to bind interleukin 2. The low-affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high-affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high-affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development. |
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