CD47 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-04810

CD47 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.3 kDa and the accession number is Q61735-1.

CD47 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-04810

CD47 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.3 kDa and the accession number is Q61735-1.

Pack Size	Price	Availability
50 μg	$228	In Stock
100 μg	$405	7-10 days
200 μg	$716	7-10 days
500 μg	$1,520	7-10 days

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COA User Guide of Recombinant Proteins

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Product Information

Biological Activity	1.Measured by its ability to bind mouse SIRPA-Fc at 10μg/mL (100μL/well) can bind mouse SIRPA-Fc, the EC50 of mouse SIRPA-Fc is 0.07-0.3μg/mL.
Description	CD47 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.3 kDa and the accession number is Q61735-1.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q61735-1
Synonyms	Itgp,IAP,CD47 molecule,B430305P08Rik,AW108519,AI848868,AA407862,9130415E20Rik
Construction	A DNA sequence encoding the mouse CD47 (Q61735-1) (Met1-Lys140) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 19
Protein Purity	> 90 % as determined by SDS-PAGE.
Molecular Weight	15.3 kDa (predicted); 34-44 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy