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Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Claudin-3 Protein-VLP, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-10xHis tag. The predicted molecular weight is 24.7 kDa and the accession number is O15551.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $815 | 20 days | |
100 μg | $1,790 | 20 days | |
1 mg | $9,870 | 20 days |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CLDN3 at 10 μg/mL can bind Anti-CLDN3 recombinant antibody, the EC50 is 23.62-34.37 ng/mL.VLPs is negative control. |
Description | Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Claudin-3 Protein-VLP, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-10xHis tag. The predicted molecular weight is 24.7 kDa and the accession number is O15551. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-10xHis (This tag can be tested only under denaturing conditions) |
Accession Number | O15551 |
Synonyms | Rat ventral prostate.1 protein homolog,hRVP1,CPETR2,CPE-receptor 2,CPE-R 2,Clostridium perfringens enterotoxin receptor 2,CLDN3,Claudin-3,C7orf1 |
Amino Acid | MSMGLEITGTALAVLGWLGTIVCCALPMWRVSAFIGSNIITSQNIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAARALIVVAILLAAFGLLVALVGAQCTNCVQDDTAKAKITIVAGVLFLLAALLTLVPVSWSANTIIRDFYNPVVPEAQKREMGAGLYVGWAAAALQLLGGALLCCSCPPREKKYTATKVVYSAPRSTGPGASLGTGYDRKDYV |
Construction | 1-220 aa |
Molecular Weight | 24.7 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 6% Trehalose, pH 7.4 |
Reconstitution | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. |
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