CNTF Protein, Mouse, Recombinant (His)

Catalog No. TMPY-00523

CNTF Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 24.7 kDa and the accession number is P51642.

CNTF Protein, Mouse, Recombinant (His)

Catalog No. TMPY-00523

CNTF Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 24.7 kDa and the accession number is P51642.

Pack Size	Price	Availability
20 μg	$212	7-10 days
100 μg	$539	7-10 days
1 mg	$3,500	7-10 days

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Product Information

Biological Activity	Measured by its binding ability in a functional ELISA. Immobilized mouse CNTF at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His . The EC50 of biotinylated rat CNTFR-His is 5-12 ng/ml.
Description	CNTF Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 24.7 kDa and the accession number is P51642.
Species	Mouse
Expression System	E. coli
Tag	N-His
Accession Number	P51642
Synonyms	ciliary neurotrophic factor,AI429687
Construction	A DNA sequence encoding the mouse CNTF (NP_740756.1) (Ala2-Met198) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: His
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	24.7 kDa (predicted); 25 kDa (reducing conditions)
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that has functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. Its actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi-subunit receptor complex, consisting of an extracellular CNTF binding subunit (CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocyteands may be relevant in reducing tissue destruction during inflammatory attacks. CNTF also is a survival factor for neurons of the peripheral sensory sympathetic and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors.