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Creatine kinase M/CKM Protein, Human, Recombinant is expressed in E. coli.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
250 μg | Inquiry | Backorder |
Biological Activity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
Description | Creatine kinase M/CKM Protein, Human, Recombinant is expressed in E. coli. |
Species | Human |
Expression System | E. coli |
Synonyms | M-CK,CPK-M,CKMM |
Protein Purity | ≥ 90% as determined by SDS-PAGE. |
Molecular Weight | 44.06 kDa (predicted) |
Endotoxin | Please contact us for more information. |
Formulation | Lyophilized from sterile 20 mM Tris, 50mM NaCl, 1mM EDTA, 5mM β-ME, 50% Glycerol, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
Reconstitution | Please refer to the lot-specific COA. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). |
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